multiple cloning site中文什么意思

多克隆位點(diǎn)

multiple: adj. 1.多重的；復(fù)合的,復(fù)式的,多數(shù)的,多樣的。 ...
clone: n. 1.【生物學(xué)】純種細(xì)胞，無(wú)性系。 2.復(fù)制品；(幾 ...
site: n. 1.地點(diǎn)；位置；地基。 2.場(chǎng)所，現(xiàn)場(chǎng)。 3.遺址 ...
cloning site: 插入位點(diǎn),克隆位點(diǎn); 克隆位點(diǎn)
multiple cloning sites: 多克隆位點(diǎn)

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例句與用法

According to csfv ' s e2 gene sequence published in genbank and the sequence of p . pastoris expression vector ppic9 ' s multiple cloning site ( mcs ) , a pair of primers were designed with oligo and primers . o softwares
將該基因片段先克隆到pmd18 - t載體上，并進(jìn)行了酶切、 pcr鑒定和測(cè)序分析，陽(yáng)性重組質(zhì)粒命名為t - e2 。
Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors , pbmb1205 , pbmb1205r , pbmb1206 and pbmb1206r . there are multiple clone sites between two copies of res sites
解離載體的構(gòu)建和性能利用來(lái)源于不同質(zhì)粒上的質(zhì)粒復(fù)制起始區(qū)ori44和ori1030構(gòu)建了4個(gè)解離載體。
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak . 8 . bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過(guò)適當(dāng)?shù)拿盖胁迦氲睫D(zhuǎn)移載體質(zhì)粒pbac - pak8的多克隆位點(diǎn)中，獲得重組轉(zhuǎn)移載體質(zhì)粒pbacpak - hbmp 。
The interest gene was inserted in the - tha l . tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system . after analysis by restriction endonuclease and pcr , the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid , the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l
同時(shí)將該目的基因插入到桿狀病毒表達(dá)系統(tǒng)的供體質(zhì)粒pf _ ( ast ) b _ ( ac ) htb的xba 、 xho多克隆位點(diǎn)間，經(jīng)酶切、 pcr鑒定后，將重組的供體質(zhì)粒gp1 - fast轉(zhuǎn)化到含有桿狀病毒和輔助質(zhì)粒的dh10b _ ( ac )感受態(tài)細(xì)胞中，獲得了表達(dá)gpvh1株vp1的重組桿狀病毒gp1 - bac 。
A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
進(jìn)一步序列分析表明該復(fù)制區(qū)至少有3個(gè)較大的orf ，分別編碼501 ， 333 ， 183個(gè)氨基酸。其中orf1蛋白序列與hd263復(fù)制蛋白o(hù)ri43的同源性為98 ，而另外兩個(gè)orf和genbank己公布的bt復(fù)制相關(guān)蛋白無(wú)同源性。 30連續(xù)培養(yǎng)72h ，復(fù)制區(qū)質(zhì)粒在bt無(wú)晶體突變株hd73cry ~ -中穩(wěn)定性達(dá)98以上， 30h生長(zhǎng)曲線也表明該復(fù)制區(qū)能夠在bt中穩(wěn)定復(fù)制和遺傳，對(duì)受體菌株無(wú)明顯不良影響。

A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites - a standard feature of engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid.

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